What can we observe an importance iris signs beside the lacunae & crypts attached on the border of the collarette and ciliary zone, constricted collarette and multiple contraction furrows around the limbus zone. Look for different layer and anger of iris, search for solitary, tiny, small and unique structure of iris signs...at IPB & inner pupillary zone.
b) Small crypts located at inner pupillary zone signify as embryological signs - 60', 125', 140' & 150' indicates genetic predisposition and family history of blood sugar imbalance, liver stress involvement, kidneys insufficiency and bladder deficiency.
c) I would suggest these tiny brown structure overlay and attached on the collarette as melanin pigments - 185', 212' & 222' it indicates predisposition to liver stress correlates with Space Risk 12 & Embryological sign at 125'. It also considered as Time Risk markings, a potential emotional trauma with deep unresolved emotional issues of anger and resentment, suppression of expression (interpretation brown pigment in emotional level) fear, worry and experiencing of violence (location of brown pigment seated on the border of the collarette - emotional dynamic of the collarette) might be happened and triggered at the age of 23~27 (for reference only). A constricted collarette indicates an inheritance predominantly by Maternal DNA or Genetic on the emotional and physical levels. In addition, the small melanin pigments located at the border of the collarette or at the topography areas of large and small intestines that firmly indicates the conflict relationship or unresolved emotional issues of anger and resentment predominant relates to mother principle !!
d) Collarette bridge at 120'~135', again it represent Time Risk marking. Please take the mid-point degree from the bridge ! Based on experienced, I would suggest most of the importance iris sign was situated inside boundary of collarette, starting from the IPB, Pupil, Inner Pupillary Zone & Border. You may place a secondary consideration for an iris signs that outside boundary of the collarette that starting from ciliary to limbus zone.